Contact an Account ManagerChromatographic separation of DNA restriction fragments
Summary
Type: HPLC
Application #: 107460
Catalog#: 736601 - MN HPLC Column (Valco type), Nucleogen DEAE, 4000A, 7um, 6x125mm
Detection: Fig. 7: UV, 260nm
Fig. 11: UV, 260nm
Conditions: Fig. 7: eluent: linear gradient from 0.5 to 0.8M NaCl in 30min, from 0.8 to 0.9M NaCl in 50min, in 30mM Na phosphate pH 6.0, 6M urea, flow rate 1.0ml/min; temperature 23°C
Fig. 11: eluent: 0.5M NaCl, 6M urea, 25 mM Na phosphate pH 6.0 for 15min, linear gradient from 0.5 to 0.7M NaCl in 40min, from 0.7 to 1.2M NaCl in 25min, in 6M urea, 25mM Na phosphate pH 6.0, flow rate 2ml/min; temperature 22°C
Authors: Hecker R
Source: J Chromatogr 418 (1987) 97 - 114
Details
Legend: Fig. 7. Anion exchange HPLC of 10µg DNA restriction fragments from the plasmid pBR 322 cleaved with restriction endonuclease HaeIII. Fragment sizes are indicated in base pairs.
Fig. 11. Anion